Mimetic Affinity Adsorbents Screening Column Kits are a range of pre-packed, re-usable columns designed to provide fast and accurate method development for most protein purification strategies. These columns are constructed using biocompatible Delrin®. They are supplied packed with 1ml volumes of high performance adsorbents in a kit form for a rapid selection of appropriate adsorbent for a given application. Once the adsorbent column is selected, it can be used for further method optimisation. Following on, the optimized conditions and the selected adsorbent column may be used to carry out a small-scale purification. Each Mimetic Affinity Adsorbent Screening Column Kit is supplied with appropriate connectors to allow the columns to be used with all the commercially available chromatography workstations. Individual columns can also be used with simple liquid delivery systems such as a peristaltic pump or syringe.

Screening column kit contains the following ten Mimetic® Affinity Adsorbents packed in 1ml volumes:

0020 Mimetic Red® 2 A6XL

0013 Mimetic Red® 3 A6XL

0030 Mimetic Orange® 1 A6XL

0040 Mimetic Orange® 2 A6XL

0050 Mimetic Orange® 3 A6XL

0060 Mimetic Yellow® 1 A6XL

0070 Mimetic Yellow® 2 A6XL

0080 Mimetic Green® 1 A6XL

3090 Mimetic Blue® 1 P6XL

3120 Mimetic Blue® SA P6XL

ADDITIONAL INFORMATION

Column - Delrin column (0.8 x 2.5 cm)

Maximum Recommended Pressure - The pressure limits specified are for the column packing material: Agarose 6XL, 7 psi/0.5 bar

pH Range - pH 2 – pH 13 (Also see adsorbent specifications)

Cleaning - Pyrogen Removal 1M NaOH

Chemical Compatibility - Compatible with most buffers, chaotropic ions and alkalis

Storage - 2-30°C in a solution of preservative, Ethanol / 0.1M NaCl (24:76 %v/v)