Hydrophobic Interaction Chromatography Adsorbent Screening Column Kits are a range of pre-packed, re-usable columns designed to provide fast and accurate method development for most protein purification strategies. These columns are constructed using biocompatible Delrin®. They are supplied packed with 1ml volumes of high performance adsorbents in a kit form for a rapid selection of appropriate adsorbent for a given application. Once the adsorbent column is selected, it can be used for further method optimisation. Following on, the optimized conditions and the selected adsorbent column may be used to carry out a small-scale purification. Each HIC Adsorbent Screening Column Kit is supplied with appropriate connectors to allow the columns to be used with all the commercially available chromatography workstations. Individual columns can also be used with simple liquid delivery systems such as a peristaltic pump or syringe.

HIC Adsorbents Screening columns are supplied in 24% Ethanol / 76% 0.1M NaCl v/v and should be stored at 2 to 30°C until required.

Product Characteristics:

Column - Delrin™ column (0.8 x 2.5 cm)

Adsorbents - Hydrophobic Interaction Chromatography Adsorbents

Maximum Recommended Pressure - The pressure limits specified are for the column packing material: Agarose 6XL, 7 psi/0.5 bar

pH Range - pH 2 – pH 13 (Also see adsorbent specifications)

Cleaning Pyrogen Removal - Up to 1M NaOH

Chemical Compatibility - Compatible with most buffers, chaotropic ions and alkalis

Storage - 2-30°C in a solution of preservative, 24% Ethanol / 76% 0.1M NaCl v/v