Literature & Downloads
Reference Publications
Development of Affinity Chromatography. Early References and Reviews: 1968 - 1976.
Selective enzyme purification by affinity chromatography. Cuatrecasas P, Wilchek M, Anfinsen CB. Proc Natl Acad Sci U S A. 1968 Oct;61(2):636-43.
Affinity chromatography. Cuatrecasas P, Anfinsen CB. Annu Rev Biochem. 1971;40:259-78.
Affinity chromatography. Parikh I, Cuatrecasas P. Vox Sang. 1972 Jul-Aug;23(1):141-6.
Affinity chromatography; a tool in protein chemistry. Wilchek M. Biochem J. 1972 Apr;127(2):7P-9P.
A new approach for the isolation of biologically active compounds by affinity chromatography: isolation of trypsin. Wilchek M, Gorecki M. FEBS Lett. 1973 Apr 1;31(1):149-52.
The literature on affinity chromatography. Wilchek M, Jakoby WB. Methods Enzymol. 1974;34:3-10.
Affinity chromatography--old problems and new approaches. March SC, Parikh I, Cuatrecasas P. Adv Exp Med Biol. 1974;42(0):3-14.
The purification of biologically active compounds by affinity chromatography. Wilchek M, Hexter CS. Methods Biochem Anal. 1976;23(0):347-85.
Development of Affinity Chromatography. Reviews:
Affinity chromatography. Wilchek M, Miron T, Kohn J. Methods Enzymol. 1984;104:3-55.
Recent advances in high-performance liquid affinity chromatography columns. Dean PD, Walker JN. Biochem Soc Trans. 1985 Dec; 13(6): 1055-8.
Recent developments in downstream processing based on affinity interactions. Luong JHT, Nguyen AL, Male KB. Tibtech 1987 (October); 5: 281-286.
New developments in affinity chromatography. Lowe CR, Burton SJ, Burton N, Stewart DJ, Purvis DR, Pitfield I, Eapen S. J Mol Recognit. 1990 Jun; 3(3): 117-22.
Separation processes in biotechnology. Process affinity chromatography. Bioprocess Technol. Clonis YD 1990;9:401-45.
A review of biotechnology and large scale affinity chromatography. Jones K. Chromatographia 1991 32 (9/10): 469-479.
Chromatography: a review of clinical applications. Hage DS. Affinity Clin Chem. 1999 May; 45(5): 593-615.
Affinity chromatography for protein isolation. Scouten WH. Curr Opin Biotechnol. 1991 Feb; 2(1): 37-43.
An overview of affinity chromatography. Wilchek M, Chaiken I. Methods Mol Biol. 2000;147:1-6.
New developments in affinity chromatography with potential application in the production of biopharmaceuticals. Lowe CR, Lowe AR, Gupta G. J Biochem Biophys Methods. 2001 Oct 30;49(1-3):561-74.
Combinatorial approaches to affinity chromatography. Lowe CR. Curr Opin Chem Biol. 2001 Jun;5(3):248-56.
Process Chromatography - Five Decades of Innovation (PDF). Curling J.M. Biopharm International, February 2007
Triazine/Dye-Ligand Specific References (not including plasma applications):
Protein purification using immobilised triazine dyes. Dean PD, Watson DH. J Chromatogr. 1979 Oct 1; 165(3): 301-19.
Affinity chromatography on immobilised triazine dyes. Studies on the interaction with multinucleotide-dependent enzymes. Clonis YD, Lowe CR. Biochim Biophys Acta. 1981 May 14; 659(1): 86-98.
Use of triazine dyes in the affinity chromatographic purification of alkaline phosphatase from calf intestine. Bouriotis V, Dean PD. J Chromatogr. 1981 Feb 27; 206(3): 521-30.
Triazine-dye affinity; chromatography. Atkinson T, Hammond PM, Hartwell RD, Hughes P, Scawen MD, Sherwood RF,Small DA, Bruton CJ, Harvey MJ, Lowe CR. Biochem Soc Trans. 1981 Aug; 9(4): 290-3.
Partial purification of anti-DNA antibodies from systemic lupus erythematosus serum by dye-ligand chromatography. Pollard KM, Webb J. J Immunol Methods. 1982 Oct 15; 54(1): 81-94.
Dye-ligand affinity chromatography: the interaction of Cibacron Blue F3GA with proteins and enzymes. Subramanian S. CRC Crit Rev Biochem. 1984; 16(2): 169-205.
Design and application of bio-mimetic dyes in biotechnology. Lowe CR, Burton SJ, Pearson JC, Clonis YD, Stead V. J Chromatogr. 1986 Apr 11;376:121-30.
The applications of reactive dyes in enzyme and protein downstream processing. Clonis YD. Crit Rev Biotechnol. 1988; 7(4): 263-79.
Chromatography on immobilized reactive dyes. Stellwagen E. Methods Enzymol. 1990; 182: 343-57.
The use of dyes in protein purification. Stead CV. Bioseparation. 1991; 2(3): 129-36.
Designer dyes: "biomimetic" ligands for the purification of pharmaceutical proteins by affinity chromatography. Lowe CR, Burton SJ, Burton NP, Alderton WK, Pitts JM, Thomas JA. Tibtech 1992; 10: 442-448.
Dye-ligand affinity chromatography. Burton SJ. Methods Mol Biol. 1992;11:1-12.
Protein purification by dye-ligand chromatography. Boyer PM, Hsu JT. Adv Biochem Eng Biotechnol. 1993; 49: 1-44.
Dye-ligand affinity chromatography on continuous beds. Mohammad J, Zeerak A, Hjerten S. Biomed Chromatogr. 1995 Mar-Apr; 9(2): 80-4.
Investigation of dye/protein interaction and its application to enzyme purification. Glemza AA. J Mol Recognit. 1990 Jun; 3(3): 137-41.
Dye-affinity techniques for bioprocessing: recent developments. Garg N, Galaev IYu, Mattiasson B. J Mol Recognit. 1996 Jul-Aug; 9(4): 259-74.
Polymer-shielded dye-affinity chromatography. Mattiasson B, Galaev IYu, Garg N. J Mol Recognit. 1996 Sep-Dec; 9(5-6): 509-14.
Dye-ligand affinity chromatography. Worrall DM. Methods Mol Biol. 1996; 59: 169-76.
Well defined dye adsorbents for protein purification. Scoble J, Scopes R. J Mol Recognit. 1996 Sep-Dec; 9(5-6): 728-32.
Biomimetic dyes as affinity chromatography tools in enzyme purification. Clonis YD, Labrou NE, Kotsira VP, Mazitsos C, Melissis S, Gogolas G. J Chromatogr A. 2000 Sep 1; 891(1): 33-44.
Dye-ligand affinity systems. Denizli A, Piskin E. J Biochem Biophys Methods. 2001 Oct 30; 49(1-3): 391-416.
Dye-ligand affinity adsorbents for enzyme purification. Labrou NE. Mol Biotechnol. 2002 Jan; 20(1): 77-84.
Affinity Chromatography - from Textile Dyes to Synthetic Ligands by Design. Part 1. Curling JM. Biopharm International. 2004 July; 17 (7): 34-42.
Affinity Chromatography - from Textile Dyes to Synthetic Ligands by Design. Part 2. Curling JM. Biopharm International. 2004 August; 17 (8): 60-66.
Triazine/Dye-Ligand Analysis, ELISAs, Safety References:
Analytical evaluation of the purity of commercial preparations of Cibacron Blue F3GA and related dyes. Hanggi D, Carr P. Anal Biochem. 1985 Aug 15; 149(1): 91-104.
Immobilized cibacron blue--leachables, support stability and toxicity on cultured cells. Hulak I, Nguyen C, Girot P, Boschetti E. J Chromatogr. 1991 Feb 22; 539(2): 355-62.
Immunochemical quantification of procion red HE-3B used as ligand in affinity chromatography. Santambien P, Girot P, Hulak I, Boschetti E. J Biochem Biophys Methods. 1992 Jun; 24(3-4): 285-95.
Development of an enzyme-linked immunosorbent assay for C.I. Reactive Blue 2 and its application to a comparison of the stability and performance of a perfluorocarbon support with other immobilised C.I. Reactive Blue 2 affinity adsorbents. Stewart DJ, Purvis DR, Pitts JM, Lowe CR. J Chromatogr. 1992; 623: 1-14.
Immunoenzymatic assay of dyes used in affinity chromatography of proteins. (Article in French) Boschetti E, Hulak I, Girot P, Santambien P. Ann Pharm Fr. 1992; 50(2): 92-102.
Immunoenzymatic assay of dyes currently used in affinity chromatography for protein purification. Santambien P, Girot P, Hulak I, Boschetti E J Chromatogr. 1992 Apr 24; 597(1-2): 315-22.
Studies of in vitro and in vivo toxicity of dyes used in affinity chromatography. (Article in French) Santambien P, Sdiqui N, Hebert E, Girot P, Hulak I, Roche AC, Monsigny M, Boschetti E. Ann Pharm Fr. 1994; 52(3): 137-52.
Toxicity studies on native Procion Red HE-3B and released dye from affinity material exposed to degradative chemical conditions. Sdiqui N, Santambien P, Roche AC, Hebert E, Girot P, Cochet S, Boschetti E, Monsigny M, Bertrand O. J Biochem Biophys Methods. 1994 Dec; 29(3-4): 269-82.
In vitro toxicity assays for dye ligands used in affinity chromatography. Santambien P, Sdiqui S, Hubert E, Girot P, Roche AC, Monsigny M, Boschetti E. J Chromatogr B Biomed Appl. 1995 Feb 3; 664(1): 241-6.
Regulatory aspects of column chromatography in biopharmaceutical manufacturing. Behizad M, Baines D. Biopharm 1998 May: 72-82.
Comparing the safety of synthetic and biological ligands used for purification of therapeutic proteins. Behizad M, Curling JM. Biopharm 2000 September; 13 (9): 42-46.
Plasma Proteins: General References on Dye-Affinity Chromatography:
The use of immobilized cibacron blue in plasma fractionation. Angal S, Dean PD. FEBS Lett. 1978 Dec 15; 96(2): 346-8.
Chromatography of plasma proteins on immobilized Cibacron Blue F3-GA. Mechanism of the molecular interaction. Gianazza E, Arnaud P. Biochem J. 1982 Jun 1; 203(3): 637-41.
A general method for fractionation of plasma proteins. Dye-ligand affinity chromatography on immobilized Cibacron blue F3-GA. Gianazza E, Arnaud P. Biochem J. 1982 Jan 1; 201(1): 129-36.
Fractionation of plasma proteins by pseudo-ligand affinity chromatography. Elution from immobilized Brown Fractogel TSK-AF. Hanahan AE, Miribel L, Arnaud P. J Chromatogr. 1987 Jun 26; 397: 197-206.
Fractionation of plasma proteins using dye-ligand chromatography: elution profiles on immobilized green TSK-AF. Miribel L, Arnaud P. J Biochem Biophys Methods. 1987 Aug; 14(5): 291-302.
The use of dye-ligand affinity chromatography for the purification of non-enzymatic human plasma proteins. Miribel L, Gianazza E, Arnaud P. J Biochem Biophys Methods. 1988 May; 16(1): 1-15.
Application of dyes in serum protein separation. Birkenmeier G, Kopperschläger G. Colloque INSERM 1989 175: 217-228
Genetically engineered and affinity purified plasma proteins. Meulien P, Tuddenham EG. Baillieres Clin Haematol. 1990 Apr; 3(2): 451-77. Review. No abstract available.
Considerations in the use of synthetic adsorbents for the manufacture of blood proteins. Burton SJ, Biotechnol Blood Proteins 1993; 227: 19-24.
Affinity-based methods for blood plasma fractionation. Curling JM. Downstream PPB 2001 Abstracts: pp 8-10. Amersham Biosciences.
Synthetic ligand adsorbents for highly selective purification of plasma proteins. Baines D, Burton M, Burton SJ, Curling JM. Recovery of Biological Products X, Cancún, Mexico. Abstracts 2001.
Affinity chromatography in the industrial purification of plasma proteins for therapeutic use. Burnouf T, Radosevich M. J Biochem Biophys Methods. 2001 Oct 30; 49(1-3): 575-86.
Plasma Proteins: Albumin - Blue References:
An Improved Performance Adsorbent for the Downstream Processing of Serum Albumin and Related Fusion Proteins Bastiaan Lobbezoo, Ben Galarza, Jim Morritt, Chloe Booth, Jennifer Walden and Dev Baines, BioProcess International, Raleigh, NC.
Selective removal of albumin from plasma by affinity chromatography. Clin. Chim. Acta 49 (1973) 49-52, Travis, J., Pannell, R.
Affinity chromatography of serum albumin with fatty acids immobilized on agarose. Peters T Jr, Taniuchi H, Anfinsen CB Jr. J Biol Chem. 1973 Apr 10;248(7):2447-51.
A method for preparing albumin-free plasma. Behring Inst. Mitt. 54 (1974) 30-32, Travis, J., Pannell, R.
Isolation of albumin from whole human plasma and fractionation of albumin-depleted plasma. Biochem. J. 157 (1976) 301-306, Travis, J., Bowen, J., Tewksbury, D., Johnson, D., Pannell, R.
Separation of human albumin by affinity chromatography. Hanford, R., d'A. Maycock, W., Vallet, L. In ‘‘Chromatography of synthetic and biological polymers, Vol. 2 Hydrophobic, ion-exchange & affinity methods''. Epton, R. (Ed.) Ellis Horwood, Cichester (1978), pp 288-292. JMC/JCC AB ProMetic-ARC: PPPS Project 25 March 2004 Page 5
Affinity chromatography on Blue dextran Sepharose of serum albumin from different animal species. Boll. Soc. It. Biol. Sper. Vol. LIV (1978) 1913-1919, Antoni, G., Botti, R., Casagli, M. C., Neri, P.
Studies on the mechanism of binding of serum albumins to immobilised cibacron blue F3GA. Biochem. J. 189 1980) 27-34, Leatherbarrow, R. J., Dean, P. D. G.
The application of affinity chromatography and hydrophobic chromatography to the purification of serum albumin. Harvey, M. J. In ‘‘ Methods of plasma protein fractionation''. Curling, J. (Ed.) Academic Press, London (1980), pp189-200.
The effect of ligand presaturation on the interactions of serum albumins with an immobilised Cibacron Blue 3G-A studied by affinity gel electrophoresis. Biochem. J. 199 (1981) 465-472, Metcalf, E., Crow, B., Dean, P. D. G.
Studies on the affinity chromatography of serum albumins from human and animal plasmas. Comp. Biochem. Physiol. 68B (1981) 319-323, Mahany, T., Khirabadi, B. S., Gersten, D. M., Kurian, P., Ledley, R. S., Ramwell, P. W.
Different interactions of human and bovine serum albumin with Cibacron Blue and Blue dextran. Ital. J. Biochem. 31 (1982) 100-106 Antoni, G., Casgli, M. C., Bigio, M., Borri, G., Neri, P.
Interactions of different albumins and animal sera with insolubilised Cibacron Blue. Evaluation of apparent affinity constants. Comp. Biochem Physiol. 71B No. 3 (1982) 403-407, Naval J., Calvo, M., Lampreave, F., Piñeiro, A.
A simple and rapid procedure for large-scale preparation of IgG's and albumin from human plasma by ion exchange and affinity chromatography. Saint-Blancard, J., Kirzin, J. M., Riberon, P., Petit, F., Fourcart, J., Girot, P., Boschetti, E. In ‘‘Affinity chromatography and related techniques'' (Gribhau, T. C. J., Visser, J., Nivard, R. J. F. Eds.) Elsevier Scientific Publishing, Amsterdam (1982) pp 305-312
The purification of albumin by affinity chromatography. Harvey, M. J., Brown, R. SA., Rott, J., Lloyd, D, Lane, R S. In ‘‘Separation of plasma proteins''. Curling, J. (Ed.) Pharmacia Fine Chemicals AB, Uppsala (1983). pp 79-88.
Application of dyes in serum protein separation. Birkenmeier, G., Kopperschläger, G. In ‘‘Biotechnology of plasma proteins''. (Stoltz, J. F., Rivat, C. Eds.) Colloque INSERM Vol. 175 (1988) Editions INSERM, Paris, 1989. pp 217-228.
Ethanol as a dispersant for Cohn Fraction IV in the large scale recovery of albumin by triazine dye affinity chromatography. More, J.E., Hitchcock, A. G., Thompson, N., Young, J. L. In ‘‘Biotechnology of plasma proteins''. (Stoltz, J. F., Rivat, C. Eds.) Colloque INSERM Vol. 175 (1988) Editions INSERM, Paris, 1989. pp 177-184.
Purification technologies for human plasma albumin. More, J. E., Harvey, M. J. In ‘‘ Blood separation and plasma fractionation'' (Harris, J. R., Ed.) Wiley-Liss, New York (1991) pp 261-306. JMC/JCC AB ProMetic-ARC: PPPS Project 25 March 2004 Page 6
Novel affinity separations based on perfluorocarbon emulsions. Use of a perfluorocarbon affinity emulsion for the purification of human serum albumin from blood plasma in a fluidised bed. McCreath GE, Chase HA, Purvis DR, Lowe CR. J Chromatogr. 1992 Apr 24; 597(1-2): 189-96.
Considerations in the use of synthetic dye-ligand affinity adsorbents for the manufacture of blood proteins. Burton, S. J. In ‘‘Biotechnology of plasma proteins''. (Stoltz, J. F., Rivat, C. Eds.) Colloque INSERM Vol. 227 (1992) Editions INSERM, Paris, 1993. pp 19-24.
All about albumin. Biochemistry, Genetics and Medical Applications. Peters, Jr., T. Academic Press, San Diego, 1996. pp 104-105.
Plasma Proteins: Immunoglobulins
Design, synthesis and application of a Protein A mimetic. Li, R., Dowd, V., Stewart, D.J., Burton, S.J., Lowe, C.R. 1998. Nature Biotechnol. 16: 190-195.
A strategy for the generation of biomimetic ligands for affinity chromatography. Combinatorial synthesis and biological evaluation of an IgG binding ligand. Teng, S.F., Sproule, K., Hussain, A., Lowe, C.R. 1999. 1999. J.Mol.Rec. 12: 67-75.
Affinity chromatography on immobilized "biomimetic" ligands. Synthesis, immobilization and chromatographic assessment of an immunoglobulin G-binding ligand. Teng, S.F., Sproule, K., Hussain, A., Lowe, C.R. 2000. J.Chromatog. B. Biomed. Sci. Appl. 740: 1-15.
Application of a synthetic adsorbent for the purification of immunoglobulins. Russell, C., Baines, D., Burton, S.J. Recovery of Biological Products IX Conference, Whistler, Canada. 23-28 May, 1999.
Immunoglobulin purification by affinity chromatography using Protein A mimetic ligands prepared by combinatorial synthesis. Kabir S. Immunol Invest. 2002 31 (3-4): 263-278.
Depletion of high abundance proteins from human plasma using a combination of an affinity and pseudo-affinity column. Urbas L, Brne P, Gabor B, Barut M, Strlic M, Petric TC, Strancar A. J Chromatogr A. 2009; 1216:2689-2694
Various Plasma Proteins: Dye-Affinity References
Lipoproteins
Interaction of Cibacron Blue and anilinonaphthalenesulphonate with lipoproteins provides a new means for simple isolation of these plasma proteins. Amthauer R, Concha M, Villanueva J, Krauskopf M. Biochem Biophys Res Comm. 1988 154 (2): 752-757.
Calcium dependent binding of the plasma protein apolipoprotein A-1 to two members of the annexin family. Brownawell AM, Creutz CE. Biochemistry 1996; 35: 6839-6845
Kallikrein
Isolation of plasma kallikrein by high efficiency affinity chromatography and its characterization. Tada M, Tsuda Y, Wanaka K, Hijikata-Okunomiya A, Horie N, Okamoto U, Okamoto S, Okada Y. Biol Pharm Bull. 2001 May; 24(5): 520-4.
a-fetoprotein
Triazine dye binding of human alpha-fetoprotein and albumin. Birkenmeier G, Usbeck E, Saro L, Kopperschlager G. J Chromatogr. 1983 Jul 29; 265(1): 27-35.
a-proteinase inhibitor
Purification of alpha 1-proteinase inhibitor by triazine dye affinity chromatography, ion-exchange chromatography and gel filtration on Fractogel TSK. Gunzer G, Hennrich N. J Chromatogr. 1984 Jul 27; 296: 221-9.
a1-acid glycoprotein
A three-step purification of human alpha 1-acid glycoprotein. Laurent P, Miribel L, Bienvenu J, Vallve C, Arnaud P. FEBS Lett. 1984 Mar 12; 168(1): 79-83.
a2-HS-glycoprotein
Interaction of alpha 2-HS-glycoprotein with immobilized triazine dyes. Arnaud P, Emerson DL, Gianazza E. Biochim Biophys Acta. 1983 Dec 28; 749(3): 270-5.
Oestrogen receptor
Estrogen receptor purification by affinity chromatography using an orange triazine dye. Bond JP, Notides AC. Anal Biochem. 1987 Jun; 163(2): 385-90. Erratum in: Anal Biochem 1987
HIV envelope glycoproteins
Triazine dyes inhibit HIV-1 entry by binding to envelope glycoproteins. Hattori T, Zhang X, Weiss C, Xu Y, Kubo T, Sato Y, Nishikawa S, Sakaida H, Uchiyama T. Microbiol Immunol. 1997; 41(9): 717-24.
Hepatitis B particles
Binding of hepatitis virus particles to immobilised procion blue-HB and cibacron blue 3GA. Brown RA, Combridge BS. J Virol Methods. 1986 Nov; 14(3-4): 267-74.
Cytokine References: Dye-Affinity/Affinity
Partial purification of human interferon by affinity chromatography. Anfinsen CB, Bose S, Corley L, Gurari-Rotman D. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3139-42.
Human lymphoblastoid interferon. Large scale production and partial purification. Bridgen PJ, Anfinsen CB, Corley L, Bose S, Zoon KC, Ruegg UT, Buckler CE. J Biol Chem. 1977 Oct 10;252(19):6585-7.
Purification and partial characterization of human lymphoblast interferon. Zoon KC, Smith ME, Bridgen PJ, zur Nedden D, Anfinsen CB. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5601-5.
Purification of proteins produced by biotechnological process. Werner RG, Berthold W. Arzneim.-Forsch. 1988; 38 (1): 422-428.
Affinity purification of recombinant interferon-? on a Mimetic Ligand adsorbent. Swaminathan S, Khanna N. Protein Expr. Purif. 1999; 15 : 236-242.
Erythropoetin; Phenylboronate References
Evaluation of phenylboronate agarose for industrial scale purification of erythropoietin from mammalian cell cultures. Zanette D, Soffientini A, Sottani C, Sarubbi E. J Biotechnol. 2003 ; 101 : 275-287.
Boronate affinity chromatography. Liu XC, Scouten WH. Methods Mol Biol. 2000;147:119-28.
Urokinase/tPA-Urokinase Fusion References
Affinity chromatography for purification of two urokinases from human urine. Takahashi R, Akiba K, Koike M, Noguchi T, Ezure Y. J Chromatogr B Biomed Sci Appl. 2000 May 26;742(1):71-8.
Development of a robust affinity adsorbent for primary capture and purification of a tPA-uokinase fusion protein. Burton SJ, Sawkins R, Pearson J, Collins S. Recovery of Biological Products XI, Banff, Canada. Abstracts 2003.
Protease References
Separation of proteases: old and new approaches. Ibrahim-Granet O, Bertrand O. J Chromatogr B Biomed Appl. 1996 Sep 20; 684(1-2): 239-63.
Affinity chromatography of serine proteases on the triazine dye ligand Cibacron Blue F3G-A. Koch C, Borg L, Skjodt K, Houen G. J Chromatogr B Biomed Sci Appl. 1998 Oct 23; 718(1): 41-6.
Affinity chromatography of plasma proteins (guanidinobenzoatase): use of mimetic matrices and mimetic soluble ligands to prevent the binding of albumin on target affinity matrices. Murza A, Aguilar AR, Fernandez-Lafuente R, Guisan JM. J. Chromatogr. B 1999 ; 732 : 165-172.
Elastase Reference
Design, synthesis and evaluation of biomimetic affinity ligands for elastases. Filippusson H, Erlendsson L, Lowe CR. J Mol Recogit. 2000; 13: 370-381.
Pyrogen References
Affinity chromatography of pyrogens. Minobe S, Shibatani T, Tosa T. Methods Mol Biol. 2000;147:155-62.
High-performance affinity capture-removal of bacterial pyrogen from solutions. J Chromatogr B Biomed Sci Appl. 2001 Aug 15;759(2):237-46.
PE40 Exotoxin Reference
The expression, affinity purification and characterization of recombinant Pseudomonas exotoxin 40 (PE40) secreted from Escherichia coli. Kawooya JK, Treat JC, Kirschner RJ, Sears MW, Gorczany JF, Grode SH, Strother DS, Asmus PA, Eckenrode FM. J Biotechnol 1995; 42: 9-22.
Affinity Precipitation References
Affinity precipitation of lactate dehydrogenase with a triazine dye derivative: selective precipitation of rabbit muscle lactate dehydrogenase with a procion blue H-B analog. Pearson JC, Burton SJ, Lowe CR. Anal Biochem. 1986 Nov 1;158(2):382-9.
Preparative affinity precipitation of L-lactate dehydrogenase. Pearson JC, Clonis YD, Lowe CR. J Biotechnol. 1989; 11: 267-274.
Dye-promoted precipitation of serum proteins. Mechanism and application. Birkenmeier G, Kopperschlager G. J Biotechnol. 1991 Nov;21(1-2):93-108.
Reversibly water-soluble affinity macroligands for bioseparation. Freitag R. Curr. Trends Polymer Sci. 1998 3: 63-79.
Affinity Membranes Review Reference
Affinity membrane chromatography for the analöysis and purification of proteins. Zou H, Luo Q, Zhou D. J Biochem Biophys Methods 2001 49: 199-240.
Aminobenzamidine References
Comparative Studies on the Inhibition of Trypsin, Plasmin and Thrombin by Derivatives of Benzylamine and Benzamadine. Markwardt ,et al., Eur. J. Biochem., 1968, Q, 502-506
Preparation of High-capacity Affinity Adsorbents Using Formyl Carriers and Their Use for low and High-Performance liquid Affinity Chromatography of Trypsin-family Proteases. Kanamori, et al., 1986, J. Chrom., 363, 231-242
Antibodies & Antibody Fragments
Application of Fabsorbent™ F1P HF, a Synthetic Ligand Adsorbent for Capture and Purification of a Single-Domain Antibody Fragement Expressed in Escherichia coli BioProcess International 7(7) (July-August 2009)
A Synthetic Ligand Affinity Adsorbent for Capture and Purification of Genetically Engineered Antibody Fragment Therapeutics Sharon Williams, Bastiaan Lobbezoo, Michelle Stobbart, Helen Kirkman and Steve Burton, BioProcess International, Raleigh, NC
Alternatives to Protein A capture (PDF) Dev Baines, Sharon Williams, Steve Burton, EuroBiotechNews Nº 3-4, Volume 8, 2009
Pathogen Removal References
The potential for low cost manufacturing of safe plasma products (PDF – Read-only), PPB09 Menorca, Spain - John Curling, Christopher Bryant, Tom Chen, Timothy Hayes, ProMetic BioTherapeutics, Inc., Neil Goss, Further Options Ltd.,Andrew Sinclair, Janice Lim, BioPharm Services Ltd., Mahesh Prashad, Sartorius Stedim Biotech
Specificity and capacity of the P-Capt® filter for prion protein and prion infectivity (PDF) Prion 2008
Reduction of prion from red cells, plasma and plasma products using specific affinity ligands (PDF) P:rion 2008
Removal of prion infectivity from plasma-derived products (PDF) Recovery of Biological Products 13 Conference
Prion Safety and Transfusion Plasma Abstract Book (PDF) 30th International Congress of the International Society of Blood Transfusion, June 7-12, 2008, Presentation by Octapharma
The introduction of a novel prion protein (PrPSc) removal technology for the pharmaceutically licensed plasma Octaplas® (PDF) 30th International Congress of the International Society of Blood Transfusion, June 7-12, 2008, Presentation by Octapharma
Biochemical quality of the pharmaceutically licensed plasma Octaplas® after implementation of a novel prion protein (PrPSc) removal technology (PDF) 30th International Congress of the International Society of Blood Transfusion, June 7-12, 2008, Presentation by Octapharma
Development of a device for prion reduction based on affinity ligand (PDF) 235th Annual Chemical Society Meeting, April 6-10, 2008. Presentation by Dr. Patrick Gurgel
Managing the risks from blood associated TSE infectivity with prion affinity ligands Part 1 (PDF) 235th Annual Chemical Society Meeting, April 6-10, 2008. Presentation by Dr. R. Rohwer
Managing the risks from blood associated TSE infectivity with prion affinity ligands Part 2(PDF) 235th Annual Chemical Society Meeting, April 6-10, 2008. Presentation by Dr. R. Rohwer
Reduction of transmissible spongiform encephalopathy infectivity from human red blood cells with prion protein affinity ligands (PDF) Luisa Gregori, Brian C. Lambert, Patrick V. Gurgel, Liliana Gheorghiu, Peter Edwardson, Julia T. Lathrop, Claudia MacAuley, Ruben G. Carbonell, Steven J. Burton, David Hammond, and Robert G. Rohwer Transfusion, Volume 46, pp. 1152-1161, July 2006
Reduction in infectivity of endogenous transmissible spongiform encephalopathies present in blood by adsorption to selective affinity resins (PDF) Luisa Gregori, Patrick V Gurgel, Julia T Lathrop, Peter Edwardson, Brian C Lambert, Ruben G Carbonell, Steven J Burton, David J Hammond, Robert G Rohwer Lancet 2006; 368: 2232-36
Prion reduction filters (PDF) Marc L Turner, Edinburgh Blood Transfusion Centre, Royal Infirmary of Edinburgh Lancet 2006; Vol 368 December 23/30