Literature & Downloads
Application Notes
Product Application Notes
Monoclonal Antibody Purification using MAbsorbent® A2P
Purification of Interferon a2b using Mimetic Blue® 1
Purification of Human Serum Albumin from Plasma using Mimetic Blue® 1
Capture of Glycoproteins by Aminophenyl Boronate Affinity Chromatography
Alkaline Phosphatase Purification using Mimetic Blue® AP
Contaminant Removal from Glucose Oxidase using Mimetic Orange® 3
Purification of Morphine Dehydrogenase using Mimetic Orange® 3
Chymosin Isolation using Mimetic Yellow® 1
Separation of Serine Proteases by para-Aminobenzamidine Affinity Chromatography
Capture and purification of recombinant albumin-fusion proteins using AlbuPure®
Optimisation of Fab fragment purification from E.coli lysate using FabsorbentTM F1P HF PuraPlateTM
Capture and purification of recombinant albumin-fusion protein using Mimetic Blue® SA HL P6XL
Fab and F(ab')2 fragment purification from CHO cell culture supernatant using FabsorbentTM F1P
Technical Posters
A novel high performance adsorbent as a platform process for the capture and purification of albumin-fusion proteins Bastiaan Lobbezoo, Richard Dodd, Phil Morton and Michael Duell
Prion reduction of blood components by affinity filtration: Performance and Clinical Experience Steve Burton and Patrick Gurgel
An Improved Performance Adsorbent for the Capture & Purification of Albumin & Albumin Related Proteins Bastiaan Lobbezoo, Chloe Booth, Jim Morritt, Jennie Walden and Steve Burton
A novel affinity adsorbent for the capture and removal of endotoxin. Bastiann Lobbezoo, Paul Beckett, Sharon Williams and Steve Burton
The Use of High Throughput Techniques for Investigating Adsorbent Batch Consistency. Chloe Booth, Bastiaan Lobbezoo, Sharon Williams
Application of in silico Techniques to the Development of Novel Ligands for Affinity Chromatography. Hassan Mamdani, Jim C. Pearson, Dev Baines, Steven J. Burton
A Comparison of Cascade Plasma Fractionation Intermediates from Site to Site and Scale-up to 30L Pools. Timothy Hayes, Tom Chen, Dev Baines, Tom Busby, Kevin Carrick, Davida Blackman and Dale Schmidt. Presented at 4th Plasma Product Biotechnology, Porto Elounda, Crete, Greece, May 9-12, 2005.
An Abbreviated Affinity Chromatography Cascade Process for Factor VIII/von Willebrand Factor Complex and Immunoglobulin G. Tom Chen, Sonday Allen, Dev Baines, Jason Betley, Davida Blackman, Timothy Hayes, Dale Schmidt, Guy Harris, Bastian Lobezoo and Keith Watson. Presented at 4th Plasma Product Biotechnology, Porto Elounda, Crete, Greece, May 9-12, 2005.
Chemspeed Generation of Combinatorial Libraries of Chromatography Media: Application to Purification of Therapeutic Proteins.Matthew J. Webb, Jim C. Pearson, Helen R. Tatton, Ben M. Beacom and Jason R. Betley. ProMetic BioSciences. abc Technologies Conference (Accelerated Bio and Chem Technologies), Basel, Switzerland, January 26 - 27, 2005.
Optimised Affinity Purification of Polyclonal Antibodies from Hyper Immunised Ovine Serum using a synthetic protein A adsorbent, MAbsorbent A2P. (Manuscript accepted 8 October, 2004, J Chromatogr B Analyt Technol Biomed Life Sci.). Anthony R. Newcombe1, Chrissie Cresswell1, Susannah Davies1, Keith Watson2, Guy Harris2, Kieran O'Donovan1, Richard Francis1. (1 - Process Development Group - Protherics Ltd.; 2 - Technical Support Group - ProMetic BioSciences Ltd.
Development of New Affinity Ligands for Primary Capture and Purification of Therapeutic Proteins. Steven J. Burton, James C. Pearson, Jason Betley, Robert Findley, Keith Watson & Dev S. Baines. Bioprocess International Conference, Boston, USA, October 4 - 7, 2004.
Purification of a Monoclonal Antibody Using The ProMetic BioSciences Monoclonal Antibody Kit.Ionela Iliescu1, Robert S. Gronke1, Jim Pearson2 and Keith Watson2. (1Biogen Idec Inc.; 2ProMetic BioSciences Ltd.). Antibody Production and Downstream Processing, San Diego, USA, February 25 - 27, 2004.
Development of a Robust Affinity Adsorbent for Primary Capture and Purification of a tPA-Urokinase Fusion Protein. Steven Burton, Richard Sawkins, Jim Pearson and Stephen Collins* (ProMetic BioSciences, *Menarini Biotech). Recovery of Biological Products XI, Banff, Alberta, Canada, September 14 - 19, 2003.
Recovery of IgG from ethanol precipitates of the Cohn-Oncley and Kistler-Nitschmann fractionaction schemes. John Curling, Dev Baines, Crystal Russell, Keith Watson, Emma Ward, Hannah Pollard and Steve Burton. Plasma Product Biotechnology Meeting, Curacao, Netherlands Antilles, 22-26 April 2003.
Production of a West Nile Virus DNA Vaccine Using Perfluorosorb S. John Ballantyne, Donald Klocke (Aldevron) and Len Smiley (ProMetic BioSciences). World Vaccine Congress, Montreal, Quebec, Canada, 7 - 9 April, 2003.
A New Chromatographic Technique for Commercial Purification of Plasmid DNA and the Simultaneous Removal of Endotoxin. Alexandra Whan, Dev Baines and Leonard Smiley. World Vaccine Congress, Montreal, Quebec, Canada, 7 - 9 April, 2003.
Design of Synthetic Ligands and their Application in Therapeutic Antibody Purification. Crystal Russell, Emma Ward, Dev Baines, Steve Burton and John Curling. 2nd International Symposium on Downstream Processing of Genetically Engineered Antibodies and Related Molecules, Porto, Portugal, 20-22 October, 2002.
Purification of Antibodies by Affinity Chromatography on "Biomimetic" Ligand Adsorbents. Dev Baines, Jim Pearson and Steve Burton. 1st International Symposium on Downstream Processing of Genetically Engineered Antibodies and Related Molecules, Barcelona, Spain, 15-17 October, 2000.
Documentation of Leakage from a Triazine Based Mimetic Affinity Matrix Developed for Purification of Coagulation Factor VIIa. Jesper Christensen, Inger Mollerup and Jens Breinholt. European Congress of Biotechnology 10, Brussels, Belgium, 11-15 July, 1999.
Comparison of Different Types of Affinity Ligands for the Purification of Coagulation Factor VIIa. Jesper Christensen, Inger Mollerup, Chris Lowe, Kenny Sproule, Paul Morrill, Steven Burton, Jim Pearson, Tad Podgorski, Alois Jungbauer and Karin Amatschek. Recovery of Biological Products IX, Whistler, British Columbia, Canada, 23-28 May, 1999.
Application of a Synthetic Ligand Adsorbent for the Purification of Immunoglobulins. Crystal Russell, Dev Baines and Steve Burton. Recovery of Biological Products IX, Whistler, British Columbia, Canada, 23-28 May, 1999.
Publications
Procedure for the Validation of Biological Active Pharmaceutical Ingredients (APIs) Manufacturing Processes. Josee Ethier. Pharmaceutical Engineering, March/April, 2005.
The Plasma Fractionation Industry. New Opportunities To Move Forward?John Curling and Christopher Bryant. BioProcess International, March, 2005.
Affinity Chromatography - from Textile Dyes to Synthetic Ligands by Design.John Curling. BioPharm International, July & August, 2004.
Purification of Plasmid DNA by Polishing and Endotoxin Removal. Victor Bornsztejn. E-Lab, June 2004.
Integrating New Technology into Blood Plasma Fractionation.John Curling. BioPharm International, September, 2002.
Comparing the Safety of Synthetic and Biological Ligands used for Purification of Therapeutic Proteins.Mehryar Behizad and John M. Curling. BioPharm International, Septmember 2000.
Presentations
The Continuing Evolution of Synthetic Ligand Affinity Adsorbents. Henry Charlton. Presented at the Antibody Development & Production Conference, Carlsbad, California, USA, March 1-3, 2006.
Overcoming New Challenges for the Downstream Purification of Antibodies. Victor Bornsztejn. Presented at the 4th Antibody World Summit, Jersey City, New Jersey, USA, July 25-27, 2005.
A New, High Yielding, Affinity Cascade for Sequential Isolation of Plasma Proteins of Therapeutic Value. Christopher Bryant, Dev Baines, Ruben Carbonell, Tom Chen, John Curling, Timothy Hayes, Steve Burton, David Hammond. Presented at 4th Plasma Product Biotechnology, Porto Elounda, Crete, Greece, May 9-12, 2005.
New adsorbents for selective capture of plasma proteins from recovered and source plasma. Jason Betley, Dev Baines, Ben Beacom, Tom Chen, Timothy Hayes, Jim Pearson, Pilar Vazquez. Presented at 4th Plasma Product Biotechnology, Porto Elounda, Crete, Greece, May 9-12, 2005.
A comparative study of Cohn and chromatographic fractionation using a novel affinity "Cascade Process".John Curling, Dev Baines, Christopher Bryant, Ruben Carbonell, Tom Chen, Patrick Gurgel, Timothy Hayes. Presented at 4th Plasma Product Biotechnology, Porto Elounda, Crete, Greece, May 9-12, 2005.
Chromatographic methods to obtain highly enriched supercoiled DNA.John Ballantyne (Aldevron LLC), Len Smiley and Victor Bornsztejn (ProMetic BioSciences Inc.). Presented at DNA Vaccines, Monte Carlo, November 17-19, 2004.
Manufacturing pDNA for Therapeutic Use. Case Study: West Nile Virus Vaccine.John Ballantyne, Michael Chambers (Aldevron LLC) and Leonard Smiley (ProMetic BioSciences Inc.). Presented at BioLOGIC USA, Boston, October 15-17, 2003.